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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. The direct fluorescent antibody (DFA) test is commonly used for the rapid detection of which respiratory pathogen directly from nasopharyngeal specimens?

A) Influenza virus
B) Bordetella pertussis
C) Legionella pneumophila (serogroup 1 in sputum)
D) Mycoplasma pneumoniae

2. A clinical microbiology laboratory is implementing a new multiplex nucleic acid amplification test (NAAT) for the detection of respiratory viral pathogens. To ensure the quantitative accuracy of the assay, it is essential to include:

A) External quality assessment samples.
B) Only positive and negative controls.
C) Calibration standards with known concentrations of target nucleic acids.
D) Internal controls to monitor for PCR inhibition.

3. A patient with recent abdominal surgery develops a fever, abdominal pain, and a wound infection. Gram stain of the wound exudate reveals a mixture of Gram-negative rods and Gram-positivebacilli. Anaerobic culture yields a foul-smelling, Gram-negative rod that is bile-esculin positive. The MOST likely organism is:

A) Bacteroides fragilis
B) Escherichia coli
C) Peptostreptococcus anaerobius
D) Clostridium perfringens

4. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Multilocus sequence typing (MLST)
B) Pulsed-field gel electrophoresis (PFGE)
C) PCR Ribotyping
D) Whole-genome sequencing (WGS) SNP analysis

5. A clinical microbiology laboratory is validating a new automated blood culture system. To assess the system's ability to detect true positive results, they should:

A) Spike known concentrations of various microorganisms into blood culture bottles.
B) Process a large number of sterile blood samples.
C) Compare the time to detection with the current manual blood culture method.
D) Evaluate the system's performance with samples from patients with known bloodstream infections.

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